TAPPI T 249 CM 2009 describes a classical laboratory method for determining the five principal monosaccharides that define the carbohydrate composition of extractive-free wood and wood pulp: glucan, mannan, arabinan, xylan, and galactan. The method covers acid hydrolysis, neutralization, reduction to alditols, acetylation, gas chromatographic analysis, calculation, calibration, reporting, and precision guidance.
This technical PDF is intended for pulp and paper laboratories, chemical analysis personnel, quality control teams, and researchers who need a preserved TAPPI procedure for carbohydrate analysis using gas-liquid chromatography. It includes apparatus and reagent requirements, sample preparation steps, calibration mixtures, calculation formulas, precision data, keywords, additional information, and appendices for chemical and chromatographic purity checks of standards.
Application Scope: Applicable to extractive-free wood and wood pulp for quantitative determination of carbohydrate constituents, with individual component concentrations as low as 0.1% determined under the stated method conditions.
