TAPPI/ANSI T 449 om-14 provides a standardized procedure for the bacteriological examination of paper and paperboard used for single-service dairy product containers and closures. The method covers required equipment and materials, sterilization, sampling, preparation of test specimens, disintegration, plating, incubation, colony counting, result reporting, and precision.
Results are reported as colony-forming units per gram of paper or paperboard. The document includes laboratory controls, dilution guidance, incubation conditions, repeatability and reproducibility information, safety considerations, and notes regarding procedural alterations such as the use of detection dyes.
Application Scope: Intended for trained laboratory personnel, paper and paperboard manufacturers, packaging quality professionals, microbiology laboratories, and organizations evaluating materials for single-service dairy product packaging.
