BS EN 1276:2009 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirements (phase 2, step 1)
BS EN 1276 is a European Standard which specifies a test method and the minimum requirements for bactericidal activity of chemical disinfectant and antiseptic products that form a homogeneous, physically stable preparation when diluted with hard water or - in the case of ready-to-use products - with water.
Products can only be tested at a concentration of 80 % or less, as some dilution is always produced by adding the test organisms and interfering substance.
BS EN 1276 applies to products that are used in food, industrial, domestic and institutional areas excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues except those for hand hygiene in the above considered areas.
Contents of BS EN 1276:
Foreword
Introduction
1􀀃 Scope
2􀀃 Normative references
3􀀃 Terms and definitions
4􀀃 Requirements
5􀀃 Test method
5.1􀀃 Principle
5.2􀀃 Materials and reagents
5.2.1􀀃 Test organisms
5.2.2􀀃 Culture media and reagents
5.3􀀃 Apparatus and glassware
5.4􀀃 Preparation of test organism suspensions and product test solutions
5.4.1􀀃 Test organism suspensions (test and validation suspension)
5.4.2􀀃 Product test solutions
5.5􀀃 Procedure for assessing the bactericidal activity of the product
5.5.1􀀃 General
5.5.2􀀃 Dilution-neutralization method
5.5.3􀀃 Membrane filtration method
5.6􀀃 Experimental data and calculation
5.6.1􀀃 Explanation of terms and abbreviations
5.6.2􀀃 Calculation
5.7􀀃 Verification of methodology
5.7.1􀀃 General
5.7.2􀀃 Control of weighted mean counts
5.7.3􀀃 Basic limits
5.8􀀃 Expression of results and precision
5.8.1􀀃 Reduction
5.8.2􀀃 Control of active and non-active product test solution (5.4.2)
5.8.3􀀃 Limiting test organism and bactericidal concentration
5.8.4􀀃 Precision, replicates
5.9􀀃 Interpretation of results - conclusion
5.9.1􀀃 General
5.9.2􀀃 Bactericidal activity for general purposes
5.9.3􀀃 Bactericidal activity for specific purposes
5.10􀀃 Test report
Annex A (informative) Referenced strains in national collections
Annex B (informative) Neutralizers and rinsing liquids
Annex C (informative) Graphical representations of dilution neutralization method and
membrane filtration method
C.1􀀃 Dilution-neutralization method
C.2􀀃 Membrane filtration method
Annex D (informative) Example of a typical test report
Test results (bactericidal suspension test)
Annex E (informative) Precision of the test result
Bibliography
