What is BS EN 16939 - Detection of tylosin, spiramycin and virginiamycin in animal feeding stuffs about?
BS EN 16939 is a European standard that serves technical specifications for the detection of tylosin, spiramycin and virginiamycin in animal feeding stuffs, enabling effective use of medicinal treatment of animals through animal feeds.
BS EN 16939 method makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feed) excluding mineral feeds and premixtures.
In the BS EN 16939 method, the limit of detection is about 2 mg/kg for spiramycin, 1 mg/kg for tylosin and 1 mg/kg for virginiamycin. In some milk replacers, it can be slightly higher than 1 mg/kg for virginiamycin. These lower limits of detection are achievable but should be established with an in-house validation first.
In the BS EN 16939 method, some other antibiotics can interfere in detecting these three specific macrolide antibiotics.
BS EN 16939 method should be used as a qualitative screening and/or a post-screening method (after microbiological plate test, for example). The follow-up of the presence of the antibiotics may be done by other analytical techniques (LC and/or LC-MS technics). For confirmatory purposes, LCMS is required.
Who is BS EN 16939 - Detection of tylosin, spiramycin and virginiamycin in animal feeding stuffs for?
BS EN 16939 on the detection of tylosin, spiramycin and virginiamycin in animal feeding stuffs applies to:
- Manufacturers of animal feeds
- Dealers and importers of animal feeds
- Testing labs for animal feeds
- Quality assurance services
- Regulatory bodies
Why should you use BS EN 16939 - Detection of tylosin, spiramycin and virginiamycin in animal feeding stuffs?
Tylosin is a clinically validated, medicinal cattle feed ingredient that helps prevent liver abscesses. Virginiamycin is usually used as a growth promoter. Spiramycin is used to enhance animal nutrition.
BS EN 16939 provides users guidance on principle, reagents, materials, apparatus, sample, measurements, determination of concentrations, precision, and test report, enabling accurate assessment and screening of compounds to ensure the quality of animal feeds.
BS EN 16939 provides users guidance on the Thin Layer Chromatography and bioautography method for detecting tylosin, spiramycin and virginiamycin in animal feeding stuffs. BS EN 16939 also provides users with a step-by-step procedure for the method, enabling users to do the technique correctly.
The adoption of BS EN 16939 allows users to assess animal feeding stuffs to detect tylosin, virginiamycin, and spiramycin, ensuring effective growth promotion and antibiotic treatment in animals.
