What is ISO 13365-1 about?
ISO 13365-1 is the first part of the multi-series ISO 13365. ISO 13365-1 specifies a test method by acetonitrile solvent extraction for the determinate one of the total contents (solvent extractible) of the following preservative agents in leather by liquid chromatography:
- 2-(thiocyanomethylthio)-benzothiazole (TCMTB)
- 4-chloro-3-methyl phenol (PCMC)
- 2-phenyl phenol (OPP)
- 2-octylisothiazol-3(2H)-one (OIT)
ISO 13365-1 can also be used to determine breakdown products of these preservative agents, which protect the leather from microbiological attacks.
Who is ISO 13365-1 for?
ISO 13365-1 on the chemical determination of the preservative in leather is useful for:
- Leather manufacturing industries
- Leather testers
- Chemical laboratories
Why should you use ISO 13365‑1?
Liquid chromatography (LC) is an analytical chromatographic technique that is useful for separating ions or molecules that are dissolved in a  solvent.
ISO 13365-1 states some specific chemicals to determine the preservatives which are as follows:
- 2-(thiocyanomethylthio)-benzothiazole (TCMTB): to control the growth of fungi and sulphate reducing bacteria
- 4-chloro-3-methyl phenol (PCMC): for maintaining low toxicity and quality performance of the product
- 2-phenylphenol (OPP): for the safety and protection purpose of the end-users
- 2-octylisothiazol-3(2H)-one (OIT): it is low toxicity, easy to operate, for protection purpose
ISO 13365-1 is beneficial for making good quality leather.
What is changed since the last update?
BS EN ISO 13365-1:2020 supersedes ISO 13365:2011. BS EN ISO 13365-1:2020includes some technical changes with respect to ISO 13365:2011. These include:
- The title has been changed to indicate the method of extraction
- The use of HPLC with mass spectrometric (MS) detection has been included
- Reagents have been technically modified.
- The chromatographic conditions (previously in 6.3) are now included in a new annex also include additional conditions for use with MS detection.
