BS EN ISO 18363-3:2021

Animal and vegetable fats and oils. Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS. Method using acid transesterification and measurement for 2-MCPD, 3-MCPD and glycidol | Status: Superseded, Withdrawn

1 Scope This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis. This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.