BS EN ISO 21676:2021

Water quality. Determination of the dissolved fraction of selected active pharmaceutical ingredients, transformation products and other organic substances in water and treated waste water. Method using high performance liquid chromatography and mass spectrometric detection (HPLC-MS/MS or -HRMS) after direct injection. | Status: Current

1 Scope This document specifies a method for the determination of the dissolved fraction of selected active pharmaceutical ingredients and transformation products, as well as other organic substances (see Table 1) in drinking water, ground water, surface water and treated waste water. The lower application range of this method can vary depending on the sensitivity of the equipment used and the matrix of the sample. For most compounds to which this document applies, the range is ≥ 0,025 µg/l for drinking water, ground water and surface water, and ≥ 0,050 µg/l for treated waste water. The method can be used to determine further organic substances or in other types of water (e.g. process water) provided that accuracy has been tested and verified for each case, and that storage conditions of both samples and reference solutions have been validated. Table 1 shows the substances for which a determination was tested in accordance with the method. Table E.1 provides examples of the determination of other organic substances. Table 1 — Substances for which a determination was tested in accordance with this method Common name Chemical name (IUPAC a ) Molecular formula Molar mass CAS-RN b g/mol 4-Acetylaminoantipyrine N-(2,3-Dimethyl-5-oxo-1-phenyl-3-pyrazolin-4-yl)acetamide C13H15N3O2 245,28 83-15-8 N4-Acetyl sulfamethoxazole N-{4-[(5-Methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl}-acetamide C12H13N3O4S 295,32 21312-10-7 Diatrizoic acid (amidotricoic acid) 3,5-Bis(acetamido)-2,4,6-triiodobenzoic acid C11H9I3N2O4 613,91 117-96-4 Atenolol (RS)-2-[4-[2-Hydroxy-3-(1-methylethylamino) propoxy]phenyl]ethanamide C14H22N2O3 266,34 29122-68-7 Bezafibrate 2-{4-[2-(4-Chlorbenzamido)ethyl]phenoxyl}-2-methylpropanoic acid C19H20ClNO4 361,80 41859-67-0 Bisoprolol (RS)-1-[4-(2-Isopropoxyethoxymethyl)phenoxy]-3-isopropylamino-2-propanol C18H31NO4 325,45 66722-44-9 Carbamazepine 5H-Dibenzo[b,f]azepine-5-carbamide C15H12N2O 236,27 298-46-4 Clarithromycin (2R,3R,4S,5R,8R,9S,10S,11R,12R,14R)-11-[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-5-ethyl-3,4-dihydroxy-9-[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyl-oxan-2-yl]oxy-12-methoxy-2,4,8,10,12,14-hexa-methyl-6-oxacyclotetradecane-1,7-dione C38H69NO13 747,95 81103-11-9 Clofibric acid 2-(4-Chlorophenoxy)-2-methylpropanoic acid C10H11ClO3 214,70 882-09-7 Dehydrato-Erythromycin (anhydro-erythromycin) (2R,3R,4S,5S,8R,9S,10S,11R,12R)-11-{[4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-5-ethyl-3-hydroxy-9-[(5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl)oxy]-2,4,8,10,12,14-hexamethyl-6,15,16-trioxatricyclo[10.2.1.1{1,4}]hexadecane-7-one C37H65NO12 715,91 23893-13-2 Diazepam (RS)-7-Chlor-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepine-2-on C16H13ClN2O 284,74 439-14-5 Diclofenac 2-[2-[(2,6-Dichlorphenyl)amino]phenyl]acetic acid C14H11Cl2NO2 296,15 15307-86-5 10,11-Dihydro-10,11-dihydroxy carbamazepine (5S,6S)-5,6-Dihydroxy-5,6-dihydrobenzo[b][1]benzazepie-11-carboxamide C15H14N2O3 270,29 58955-93-4 Erythromycin 6-(4-Dimethylamino-3-hydroxy-6-methyl-oxan-2-yl)oxy-14-ethyl-7,12,13-trihydroxy-4-(5-hydroxy-4-methoxy-4,6-dimethyl-oxan-2-yl)-oxy-3,5,7,9,11,13-hexamethyl-1-oxacyclotetradecane-2,10-dione C37H67NO13 733,93 114-07-8 4-Formylaminoantipyrine N-(2,3-Dihydro-1,5-dimethyl-3-oxo-2-phenyl-1H-pyrazol-4-yl)formamide C12H13N3O2 231,25 1672-58-8 Gemfibrozil 5-(2,5-Chlorophenoxy)-2,2-methylpropanoic acid C15H22O3 250,34 25812-30-0 Ibuprofen (RS)-2-[4-(2-Methylpropyl)phenyl]propanoic acid C13H18O2 206,28 15687-27-1 Iomeprol (±)-N,N′-Bis-(2,3-dihydroxypropyl)-5-[(2-hydroxy-acetyl)methylamino]-2,4,6-triiodo isophthalamide C17H22I3N3O8 777,09 78649-41-9 Iopamidol (S)-N,N′-Bis[2-hydroxy-1-(hydroxymethyl)ethyl]-5-[(2-hydroxypropanoyl)amino]-2,4,6-triiodobenzene-1,3-dicarbamide C17H22I3N3O3 777,08 60166-93-0 Iopromide (±)-N,N′-Bis(2,3-dihydroxypropyl)-2,4,6-triiodo-5-(2-methoxyacetamido)-N-methylisophthalamide C18H24I3N3O8 791,12 73334-07-3 Metoprolol (RS)-1-(Isopropylamino)-3-[4-(2-methoxyethyl) phenoxy]propan-2-ol C15H25NO3 267,36 37350-58-6 Naproxen (S)-2-(6-Methoxy-2-naphthyl)propanoic acid C14H14O3 230,26 22204-53-1 Oxazepam (RS)-7-Chloro-3-hydroxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-on C15H11ClN2O2 286,71 604-75-1 Phenazone 1,5-Dimethyl-2-phenyl-2,3-dihydro-1H-pyrazol-3-on C11H12N2O 188,23 60-80-0 Primidone 5-Ethyl-5-phenylhexahydropyrimidin-4,6-dione C12H14N2O2 218,25 125-33-7 Propyphenazone 1,5-Dimethyl-4-(1-methylethyl)-2-phenyl-1,2-dihydro-3H-pyrazol-3-one C14H18N2O 230,31 479-92-5 Roxithromycin (3R,4S,5S,6R,7R,9R,11S,12R,13S,14R)-6-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-14-ethyl-7,12,13-trihydroxy-4-{[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl]oxy}-3,5,7,9,11,13-hexamethyl-10-(2,4,7-trioxa-1-azaoctan-1-ylidene)-1-oxacyclotetradecane-2-one C41H76N2O15 837,05 80214-83-1 Sotalol (RS)-4′-(1-Hydroxy-2-isopropylaminoethyl) methanesulfonanilide C12H20N2O3S 272,36 3930-20-9 Sulfamethoxazole 4-Amino-N-(5-methyl-1,2-oxazol-3-yl)benzene-sulfonamide C10H11N3O3S 253,28 723-46-6 Temazepam (RS)-7-Chloro-3-hydroxy-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one C16H13ClN2O2 300,74 846-50-4 Trimethoprim 2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine C14H18N4O3 290,32 738-70-5 a IUPAC: International Union of Pure and Applied Chemistry. b CAS-RN: Chemical Abstracts System Registration Number.