What is ISO 9167-Determination of glucosinolates content in rapeseed and rapeseed meals about?
ISO 9167 discusses rapeseed and rapeseed meals. ISO 9167 is an international standard on the determination of glucosinolates content in rapeseed and rapeseed meals using high-performance liquid chromatography to ensure the prevention of various health hazards.
ISO 9167 specifies a method for the determination of the individual glucosinolates content in rapeseed and rapeseed meals using high-performance liquid chromatography with gradient elution.
ISO 9167 specifies a method using high-performance liquid chromatography (HPLC) with gradient elution as a reference method. For the isocratic mode, the choice of the internal standard, the chromatographic conditions, and the separation results are different from the reference method.
The method under ISO 9167 was tested on rapeseed and rapeseed meals (Brassica rapa, Brassica napus, and Brassica juncea) but applies to other plant materials, on the condition that the occurring glucosinolates previously identified are described in ISO 9167.
Note: The method specified under ISO 9167 does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.
Who is ISO 9167-Determination of glucosinolates content in rapeseed and rapeseed meals for?
ISO 9167 on the determination of glucosinolates content in rapeseed and rapeseed meals is useful for:
- Food scientists
- Food laboratories
- Food technologists
- Food health and safety departments
Why should you use ISO 9167-Determination of glucosinolates content in rapeseed and rapeseed meals?
Glucosinolates are anti-nutrient factors that have been demonstrated to cause feed intake reduction, iodine deficiency, and hypertrophy of organs such as the liver, kidney, and thyroid gland.
ISO 9167 provides you with methods, reagents, apparatus, sampling, procedures. ISO 9167 describes a chromatographic method with two conditions (gradient and isocratic) of elution for qualitative and quantitative analysis of individual glucosinolates in rapeseed and rapeseed meals.
The methods and procedures provided by ISO 9167 ensure the reduction of the risk of fungal infection in the intestines and other parts of the body.
By adopting ISO 9167 the user can determine the glucosinolates content in rapeseed and rapeseed meals correctly and efficiently.
What is changed since the last update?
BS EN ISO 9167:2019 supersedes ISO 9167‑1:1992, which is withdrawn. BS EN ISO 9167:2019 has made some technical changes with respect to ISO 9167‑1:1992. These include:
- Rapeseed meals have been added to the scope with the addition of a new collaborative trial
- In 9.2, methanol 70 % has been replaced by ethanol 50 % for lower toxicity and only one extraction is carried out instead of two
- In 10.2 and E.5.1, the term “relative proportionality factor” has been used instead of “response factor”
- The isocratic mode has been added in Annex E
