This International Standard specifies a method for determining the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to
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chemical substances and preparations,
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marine and brackish waters,
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streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected, and
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aqueous extracts (pore water, elutriates, eluates, and leachates) from sediments and petroleum products.
