BS ISO 20397-3:2025

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:

1. sampling strategy and process, including type, storage, transportation, extraction, quality;

2. nucleic acid library preparation

3. design and review process including sequencing strategy and assessment;

4. database construction;

5. bioinformatics analysis and report

6. validation and verification for bioinformatics pipeline, and database

This document applies to laboratories and research organizations.