DD ISO/TS 21872-2:2007

Microbiology of food and animal feeding stuffs. Horizontal method for the detection of potentially enteropathogenic Vibrio spp.. Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae | Status: Superseded, Withdrawn

1 Scope This part of ISO/TS 21872 specifies a horizontal method for detection of the enteropathogenic Vibrio species, causing illness in or via the intestinal tract, other than Vibrio parahaemolyticus and Vibrio cholerae. The species detectable by the methods specified include Vibrio fluvialis, Vibrio mimicus and Vibrio vulnificus1). It is not suitable for the isolation of Vibrio hollisae. Strains of V. parahaemolyticus and V. cholerae may also be detected during the application of this method. This part of ISO/TS 21872 is applicable to

• — products intended for human consumption and the feeding of animals, and
• — environmental samples in the area of food production and food handling.

This method is not appropriate for the detection of Vibrio metschnikovii as this is oxidase negative.

NOTE 1 Vibrio metschnikovii has been occasionally isolated from human faecal samples and can be a cause of diarrhoeal diseases.

NOTE 2 The identification of Vibrio species other than V. parahaemolyticus and V. cholerae is difficult, and needs further development. The biochemical tests given in this part of ISO/TS 21872 enable only a presumptive confirmation of these species.

NOTE 3 Reasons for not applying this method are discussed in the Introduction.