What is PD CEN/TS 17303– DNA barcoding for fish about?
PD CEN/TS 17303 describes a procedure for the identification of single fish and fish fillets to the level of genus or species.
The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI) [2], [3] or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases. The methodology allows the identification of a large number of commercially important fish species.
PD CEN/TS 17303 is usually unsuitable for the analysis of highly processed foods, e.g., tins of fish, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex fish products containing mixtures of two or more fish species.
Who is PD CEN/TS 17303– DNA barcoding for fish for?
PD CEN/TS 17303 on DNA barcoding of fish is relevant to:
- Fish dealers and suppliers
- Manufacturers of fish products
- Microbiologists
- Health and safety department in fish industries
Why should you use PD CEN/TS 17303– DNA barcoding for fish?
DNA barcoding method uses a short, standardized genetic region to act as a "barcode", which is used to identify species.
PD CEN/TS 17303 provides methods and procedures for barcoding fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments.
The methods described in PD CEN/TS 17303 has been successfully validated on raw fish fillets, however, laboratory results suggest that it can also be applied to processed, e.g., cold smoked, hot smoked, salted, frozen, cooked, fried, deep-fried samples.
Ultimately, PD CEN/TS 17303 provides methods for barcoding fish items using DNA segments which will simplify business operations and increase shelf life and quality by providing a more accurate way to recognize better quality fish.
