What is ISO/TS 12869 - Detection and quantification of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification about?
ISO/TS 12869 is a technical specification on water quality that discusses the detection and quantification of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification by quantitative polymerase chain reaction (qPCR) to enhance the water quality.
ISO/TS 12869 specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It also specifies general methodological requirements, performance evaluation requirements, and quality control requirements.
ISO/TS 12869 is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.
The method under ISO/TS 12869 applies to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.
Note: The qPCR methods do not give any information about the physiological state of Legionella.
Who is ISO/TS 12869 - Detection and quantification of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification for?
ISO/TS 12869 on the detection and quantification of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification are useful for:
- Microbiologists
- Hydrologist
- Microbiology laboratory
- Water regulatory corporations
Why should you use ISO/TS 12869 - Detection and quantification of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification?
The presence of L. pneumophila or Legionella spp. in water samples is demonstrated and quantified by amplifying DNA sequences (PCR) with specific oligonucleotides. The specificity of the detection is ensured by using a target sequence-specific fluorescent-labelled probe. The increase in the amount of the DNA amplicon can be measured and visualized in real-time by a quantitative PCR device with fluorophore-specific filters.
ISO/TS 12869 specifies a determination of the recovery of the DNA extraction.
ISO/TS 12869 provides you with method, general methodological requirements, performance evaluation requirements, and quality control requirements.
This assists in the efficient detection and evaluation of Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification.
With compliance and obedience to ISO/TS 12869, you can properly detect Legionella spp. and/or Legionella pneumophilaby concentration and genic amplification to enhance the quality of water.
What’s changed since the last update?
PD ISO/TS 12869:2019 supersedes ISO/TS 12869:2012, which is withdrawn, PD ISO/TS 12869:2019 has made some technical changes compared to ISO/TS 12869:2012. These includes:
- Meet expectations from customers and governments faced with Legionella risk
- The use of new technologies while overseeing the development work of various actors in the sector has been allowed
- The return of experiences from the laboratories using this method since 2006 has been taken into account
- In Annex G, information on the evolution of the requirements for the use of the third party validated commercial kits has been added.
