PD ISO/TS 21569-3:2015

Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products. Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms | Status: Superseded, Withdrawn

1 Scope This Technical Specification describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis has to be carried out. This Technical Specification is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.